Hi I'm running into some unexpected behaviour whilst try to use an SA fibre of a QGram index that's built over a string set. The below code runs OK with 1 or 10 as command line args, (strings in string set), but fails at 100 with the following exception: /Users/dj5/usr/local/include/seqan/sequence/string_base.h:238 Assertion failed : static_cast<TStringPos>(pos) < static_cast<TStringPos>(length(me)) was: 171599218 >= 100 (Trying to access an element behind the last one!) Have I made a coding blunder or is this a bug? Many thanks, Daniel <code> #include <ctime> #include <cstdlib> #include <iostream> #include <sstream> #include <string> #include <algorithm> #include <seqan.h> using namespace std; using namespace seqan; // Generates random nucleotides. struct MyGenerator : public unary_function<char, void> { string syms; MyGenerator (string syms = "ACGT") : syms(syms) { srand(time(NULL)); } char operator()(void) { return syms[rand() % syms.size()]; } }; int main(int argc, char** argv) { // Input the number of sequences for the string set. stringstream ss(argv[1]); unsigned n; ss >> n; typedef StringSet<DnaString> TMyStringSet; typedef Index<TMyStringSet, IndexQGram<UngappedShape<8> > > TMyIndex; typedef Fibre<TMyIndex, QGramSA>::Type TMySAFibre; typedef Fibre<TMyIndex, QGramDir>::Type TMyDirFibre; // Fill a string set with 60-mer DNA sequences. StringSet<DnaString> myStringSet; string input_s; DnaString input; for (unsigned i = 0; i < n; ++i) { input_s.resize(60); generate_n(input_s.begin(), 60, MyGenerator()); input = input_s; appendValue(myStringSet, input); } // Build the index. TMyIndex index(myStringSet); // Require the QGramSA fibre. cout << "requiring SA fibre:\n"; float t0 = clock(); indexRequire(index, QGramSA()); cout << (clock() - t0)/CLOCKS_PER_SEC << endl; cout << "requiring Dir fibre:\n"; t0 = clock(); indexRequire(index, QGramDir()); cout << (clock() - t0)/CLOCKS_PER_SEC << endl; TMySAFibre mySAFibre = getFibre(index, QGramSA()); cout << "QGramSA length: " << length(mySAFibre) << endl; TMyDirFibre myDirFibre = getFibre(index, QGramDir()); cout << "QGramDir length: " << length(myDirFibre) << endl; return 0; } </code> -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE.