Re: [Seqan-dev] splazers cigar mismatch

["Holtgrewe, Manuel" <manuel.holtgrewe@fu-berlin.de>]

Can you also give me the command lines you are using?
________________________________________
From: Tina Hu [t.hu@celmatix.com]
Sent: Wednesday, June 20, 2012 4:20 PM
To: seqan-dev@lists.fu-berlin.de
Subject: Re: [Seqan-dev] splazers cigar mismatch

Hi Manuel,

Below are the sequences that produce this mismatch. This is run against chr1 of hg19.

Thanks!
Tina

@truncated
AAATTGCGTTTAAATTCTTCCCTGGAGGCAGAACACTAAATCCTTTTGTAA
TTATTTACAGCTCACATCCTTAGAGCTGCAATATGTTTCGCCATAACTTCTGATGTGCCG
GGACCTTGAATTGGCTGTGCCAGGAACATATGACCCAACCNGCCAATCATTCGCATTCAG
TCCATAG
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIII
@full
GTGCCTGCTTTCTCGTCTCAGGCCTATGGTCGAGATTTAATGGAGGCCCAAGAGTGGTGC
AGGAAGTACATGAAATCAGGGAATGTCAAGGACCTCACGCAAGCCTGGGACCTCTATTAT
CATGTGTTCCGACGAATCTCAAAGCAGCTGCCTCAGGTAGGATCTTCAGGCTCCTGGCAG
GGTTAACTGTCATTATAGTCCTTTCTGTTTTACTGTCTATGTAATTGTTCTAACACTCAT
TCCAAAGCATCTGGTTTTACTCTGCTTTGGGACAAGTAATTGTTATTAGCAATCCACTGA
AAAATCTTAAAATTGCGTTTAAATTCTTCCCTGGAGGCAGAACACTAAATCCTTTTGTAA
TTATTTACAGCTCACATCCTTAGAGCTGCAATATGTTTCGCCAAAACTTCTGATGTGCCG
GGACCTTGAATTGGCTGTGCCAGGAACATATGACCCAACCNGCCAATCATTCGCATTCAG
TCCATAG
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
IIIIIII

--
Tina Hu, Ph.D.
Bioinformatics Scientist
Celmatix, Inc.
1 Little West 12th St.
New York, NY 10014
917.426.2878

www.celmatix.com

This email message and any attachments being sent by Celmatix, Inc., are confidential, and may be privileged. If you are not the intended recipient, please notify us immediately—by replying to this message—and destroy all copies of this message and any attachments. Thank you.

On Jun 20, 2012, at 6:00 AM, seqan-dev-request@lists.fu-berlin.de wrote:

> Today's Topics:
>
>   1. splazers cigar mismatch (Tina Hu)
>   2. Re: splazers cigar mismatch (Holtgrewe, Manuel)
>
> ------------------------------
> Message: 2
> Date: Wed, 20 Jun 2012 05:15:29 +0000
> From: "Holtgrewe, Manuel" <manuel.holtgrewe@fu-berlin.de>
> To: SeqAn Development <seqan-dev@lists.fu-berlin.de>
> Subject: Re: [Seqan-dev] splazers cigar mismatch
> Message-ID:
>       <FCCAB9D80C3DAB47B5601C5B0E62872B0A2D1F@ex02a.campus.fu-berlin.de>
> Content-Type: text/plain; charset="windows-1252"
>
> Dear Tina,
>
> could you provide us with the whole problematic record?
>
> Cheers,
> Manuel
>
> ________________________________
> From: Tina Hu [t.hu@celmatix.com]
> Sent: Tuesday, June 19, 2012 9:13 PM
> To: seqan-dev@lists.fu-berlin.de
> Subject: [Seqan-dev] splazers cigar mismatch
>
> Hi,
>
> I get the following error from snp_store (on output from splazers):
> WARNING! Read TESTING: cigar alignment length does not match genome coordinates. Discarding read..
>
> I'm fairly certain this error stems from splazers as the alignment length computed from the cigar string does not match the start and end match position (off by 1 bp). Interestingly, when I truncate 300 bps same sequence from 478 bps to 178 bps, the cigar string appears to be correct. Note that 32M is now 31M which is the correct length.
>
> cigar of the longer sequence (478 bps):
> 14M1I206M1I2M1I35M1D45M1D3M1I147M1D32M
>
> cigar of the truncated sequence (178 bps):
> 147M1D31M
>
> Has anyone else encountered this issue and is there a fix?
>
> Thanks!
> Tina
>
> --
> Tina Hu, Ph.D.
> Bioinformatics Scientist
> Celmatix, Inc.
> 1 Little West 12th St.
> New York, NY 10014
> 917.426.2878
>
> www.celmatix.com<http://www.celmatix.com>
>
> This email message and any attachments being sent by Celmatix, Inc., are confidential, and may be privileged. If you are not the intended recipient, please notify us immediately?by replying to this message?and destroy all copies of this message and any attachments. Thank you.
>
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