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[Seqan-dev] rabema_prepare_sam "File must be sorted by query name."

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  • From: Uwe Appelt <uappelt@clcbio.com>
  • To: SeqAn Development <seqan-dev@lists.fu-berlin.de>
  • Date: Tue, 11 Sep 2012 11:10:00 +0200
  • Reply-to: SeqAn Development <seqan-dev@lists.fu-berlin.de>
  • Subject: [Seqan-dev] rabema_prepare_sam "File must be sorted by query name."

Hi guys,

I've got a small problem in Rabema: I am preparing another gold standard for a Rabema benchmark and received the following error msg:

./razers3 -v -tc 32 -of sam -rr 100 -i 90 -m 1000000 -ds -o ./output0.sam ./hg19.fa ./input.fastq
[load of outputs]
./rabema_prepare_sam ./output0.sam > ./output.sam

ERROR: 16A7I:4:100 succeeds 16A7I:49:92 in SAM file.
File must be sorted by query name.

Seems like razers3 and rabema_prepare_sam are somehow out of sync with respect to their sorting code (maybe it's just the multi-threading in razers3 that sometimes results in slightly "unordered" output of mapping results)? The Trunk-checkout is, however, already a week old, so I might have to re-run everything. Before doing so, I wanted to ask for advice here, because re-running razers3 will take 4 days. Any ideas or suggestions?

Thanks in advance,
Uwe



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    • Re: [Seqan-dev] rabema_prepare_sam "File must be sorted by query name."
      • From: "Holtgrewe, Manuel" <manuel.holtgrewe@fu-berlin.de>
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