I am getting stuck on the simplest of tasks, reading an uncompressed fastq file. This statement: seqan::SequenceStream seqIO(argv[1]); returns a FILE_FORMAT_ERROR on a valid fastq text file. In sequence_stream.h in the function _checkFormat, checkStreamFormat returns false, // Guess file format, given the record reader. template <typename TStream, typename TSpec> SeqIOFileFormat_::Type _checkFormat(RecordReader<TStream, TSpec> & recordReader) { AutoSeqStreamFormat formatTag; if (!checkStreamFormat(recordReader, formatTag)) return SeqIOFileFormat_::FILE_FORMAT_ERROR; The recursion in checkStreamFormat in guess_stream_format.h is confusing me so I can't figure out what specific failure it is tripping over. I am trying to debug this but need some help deciphering the recursion architecture of the guessFormat code. How do I know it is a valid fastq file? The file provided has been a test file for non seqan code, and I have been able to use it in seqan with the FragmentStore without trouble.