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[Seqan-dev] Fastq test files

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  • From: Theodore Omtzigt <theo@stillwater-sc.com>
  • To: SeqAn Dev List <seqan-dev@lists.fu-berlin.de>
  • Date: Fri, 01 Mar 2013 18:00:11 -0500
  • Organization: Stillwater Supercomputing, Inc.
  • Reply-to: SeqAn Development <seqan-dev@lists.fu-berlin.de>
  • Subject: [Seqan-dev] Fastq test files

I just got a set of FASTQ test files from Illumina BaseSpace and SeqAn is barfing on them reporting INVALID_FORMAT.

s_G1_L001_I1_001.fastq.1,
s_G1_L001_I1_002.fastq.1,
s_G1_L001_R1_001.fastq.1,
s_G1_L001_R1_002.fastq.1,
s_G1_L001_R2_001.fastq.1,
s_G1_L001_R2_002.fastq.1
Here is a quick snippet of the first file

@:89:A0172:1:1:12008:1323 1:N:0:1
TTAGGC
+
;B@FFF
@:89:A0172:1:1:15627:1329 1:N:0:1
TTAGGC
+
@CCFFF
@:89:A0172:1:1:19263:1331 1:N:0:1
TTAGGC
+
@@CDDF
@:89:A0172:1:1:24249:1331 1:N:0:1
TTAGGC
+
BCCFFF
@:89:A0172:1:1:15721:1332 1:N:0:1
TTAGGC
+
<@<DAD
@:89:A0172:1:1:15433:1333 1:N:0:1
TTAGGC


Would it be possible to include a couple of very short test files in the SeqAn src tree, say under seqan/data, that do pass successfully through readRecord() so that software development can continue while I/O issues are sorted out?

Would also be nice to know why these Illumina BaseSpace files don't pass.
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  • Follow-Ups:
    • Re: [Seqan-dev] Fastq test files
      • From: "Holtgrewe, Manuel" <manuel.holtgrewe@fu-berlin.de>
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