Manuel: using your pointer that Dna/Dna5 discrepancies will error, I
was able to found the error: the files contain a couple of records
that contain 'N' characters: making the shortReads a
seqan::String<Dna5> solves our problem. SeqAn is AWESOME! ... @:89:A0172:1:1:24794:29480 1:N:0:1 CTTAAAAACATACCGTGTCCCCCGAGCTGTACCATTCCTGCTTCAAAAGATACGCGAGTACGAGTTGAATGTTTATCAAAGATAAGCCCTAATATTTTGCCTTGCAGTAAAGGCTCTTGCTTATTCTTTTTTAAATATATTGCGAACTCAA + CCCFFFFFGHHHHJJIGIIIIJJJJJJJFGHIIJFIJJJJJIJJJIJJEIIIIGIJHF>BACDDD@@>CCA:>ADDCEDACDDDDCCCDDDDDDDD>C@CCDD:@@CCCD<@:?:?AC@@C::@DEEDCDDDDDCADDC:B:399@>B>:+ @:89:A0172:1:1:18943:29511 1:N:0:1 ATTCTTGATTGTCCGATACCACCGCCAATTGTTAATGGGAGTACGTCTTCTAAAATACCTTTATGGAAATCGTACTCACGTTTGAAGTCTTCACCTGTTTTCGTTAACTGCTCATCAAGTGATTTACTATCAACACGAATTCCCATTGATG + CCCFFFFFGHHHHJJIJIJJIIJDIJJJJJJGJIJIIJJJJJIIECGEIGJJJIFIJIHHHHHHHFFFFFED=ABBDDDDDDCDDDC@CDDDCDDDC>CCDDDDCBBCCCDCDDDDCCA@ACDEDDEDDECDDDDD@9&(8:>CDDDCDCA @:89:A0172:1:1:19103:29594 1:N:0:1 NTCAATTGAGTATCCTTTCGCAAGCCCTGCTGTAACTGCTGAAGCAAATGTACACCCGCTTCCATGCGTTTGCTTTGAAGGAATTCGTTCACTTTTAAATTCAATAAACTTTTCTCCATCAAAAAGTAAATCAATTACTTCATTACCTTGA + #114422232222222223343342211111111111000000000000000110.-.----.....-,,,,....-----,-,,,,,,,,,,,++++++++++++(++,++++++++,++++(+)+++++++++++++++++++(++++( @:89:A0172:1:1:19050:29614 1:N:0:1 NCAATATGGGAATTTTGCTTCTCGAACAAGGGGGTGATCCGCTTTTTCTGGAACGAGAATTGTGATTTGAACCCCGCGTTTTCGTGCTTCTAATAATGCATTCATAATTTTTCTTCCAGGAATGAAATATGGTGTACCGATAAAGAGTTCT + #14442222222232223333333342221111000..//.----.........,,,,,..---------,,,,,,)))))+,)++++++++++++++++++++++++++++++++(+++++++++,+++++++++++))))+++++++,+ @:89:A0172:1:2:17834:1321 1:N:0:1 TCAAACAGCATGGGCGTTAGATGCTCTCATTTCTTACTATGATAAAGAAACACCAACTATTCGAAAAGGCGTTTCATATTTGCTTGCGAATCCTTATGTGAATGAAAAATATCCTACTGGAACAGGTTTACCTGGTGGGTTTTATATTCGT + <@@FFFFFFHHHHIDHGIGGHIJFHIIHJIIGGGIG@DDFHCIGIIGIJJGIBFEIGIIJIJIJJFGHHFEDDBCEEEDEEACDDC?@BDDBCCCCCDDEED>@CCCDDBDDDEDD@CDDCAADCBDCCCC@CDD:9?B&2(2:AA>@B<A @:89:A0172:1:2:16184:1324 1:N:0:1 TTAAAACCATGTTAATATATTTTTGTATAATTAAGAATTTAATTTACAAGGGGGATTATATATGTTCAAAAAAGTAGCTGCTGATGTTTTAGGGTTAAGTGATGTAGGTTCTGTCATTACACCGAAAGATTATGACAAGGTCGATGCTGAT + ?@@FDDDDDHDFHIIIGGGIHIIGGFIIGIIIIIIFEIIIIIGGIIIIGHIGIIHHIIIGIIIIIDGCEGEEFF@CECEECDDD;>>CCCDDDDCBDCAACDEDBACCC@CDDEDDDEEDAAA@BBDDCDDDCD@>>A8?:()0<ABCCD@ |