Would you let me know which local alignment algorithm support scoring matrix such as blosum62? If any, can you show me a function call or an example? I am using seqan 1.3. I am attaching error messages from local alignment call at the end of the message. Thanks, Youngik. /home/yyang/bin/seqan/seqan/graph_align/graph_align_smith_waterman.h: In function 'typename seqan::Value<TRight, 0>::Type seqan::_alignSmithWaterman(TTrace&, const TStringSet&, const TScore&, typename seqan::Value<THolder, 0>::Type&, TIndexPair&, TForbidden&) [with TTrace = seqan::String<unsigned char, seqan::Alloc<void> >, TStringSet = seqan::Align<seqan::String<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Alloc<void> >, seqan::ArrayGaps>, TScore = seqan::Score<int, seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_> >, TIndexPair = size_t [2], TForbidden = seqan::Nothing]': /home/yyang/bin/seqan/seqan/graph_align/graph_align_smith_waterman.h:381: instantiated from 'typename seqan::Value<TLeft, 0>::Type seqan::_localAlignment(const TStringSet&, const TScore&, seqan::SmithWaterman) [with TStringSet = seqan::Align<seqan::String<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Alloc<void> >, seqan::ArrayGaps>, TScore = seqan::Score<int, seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_> >]' /home/yyang/bin/seqan/seqan/graph_align/graph_align_interface.h:378: instantiated from 'TScoreValue seqan::localAlignment(const TStringSet&, const seqan::Score<TScoreValue, TScoreSpec>&, TTag) [with TStringSet = seqan::Align<seqan::String<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Alloc<void> >, seqan::ArrayGaps>, TScoreValue = int, TSpec = seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_>, TTag = seqan::Tag<seqan::SmithWaterman_>]' alignment_local.cpp:65: instantiated from here /home/yyang/bin/seqan/seqan/graph_align/graph_align_smith_waterman.h:233: error: no match for 'operator[]' in 'str[0]' /home/yyang/bin/seqan/seqan/graph_align/graph_align_smith_waterman.h:234: error: no match for 'operator[]' in 'str[1]' /home/yyang/bin/seqan/seqan/score/score_base.h: In function 'TValue seqan::score(const seqan::Score<TValue, TSpec>&, TPos1, TPos2, const TSeq1&, const TSeq2&) [with TValue = int, TSpec = seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_>, TPos1 = long unsigned int, TPos2 = long unsigned int, TSeq1 = seqan::SimpleType<unsigned char, seqan::AminoAcid_>, TSeq2 = seqan::SimpleType<unsigned char, seqan::AminoAcid_>]': /home/yyang/bin/seqan/seqan/graph_align/graph_align_smith_waterman.h:291: instantiated from 'typename seqan::Value<TRight, 0>::Type seqan::_alignSmithWaterman(TTrace&, const TStringSet&, const TScore&, typename seqan::Value<THolder, 0>::Type&, TIndexPair&, TForbidden&) [with TTrace = seqan::String<unsigned char, seqan::Alloc<void> >, TStringSet = seqan::Align<seqan::String<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Alloc<void> >, seqan::ArrayGaps>, TScore = seqan::Score<int, seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_> >, TIndexPair = size_t [2], TForbidden = seqan::Nothing]' /home/yyang/bin/seqan/seqan/graph_align/graph_align_smith_waterman.h:381: instantiated from 'typename seqan::Value<TLeft, 0>::Type seqan::_localAlignment(const TStringSet&, const TScore&, seqan::SmithWaterman) [with TStringSet = seqan::Align<seqan::String<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Alloc<void> >, seqan::ArrayGaps>, TScore = seqan::Score<int, seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_> >]' /home/yyang/bin/seqan/seqan/graph_align/graph_align_interface.h:378: instantiated from 'TScoreValue seqan::localAlignment(const TStringSet&, const seqan::Score<TScoreValue, TScoreSpec>&, TTag) [with TStringSet = seqan::Align<seqan::String<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Alloc<void> >, seqan::ArrayGaps>, TScoreValue = int, TSpec = seqan::ScoreMatrix<seqan::SimpleType<unsigned char, seqan::AminoAcid_>, seqan::Blosum62_>, TTag = seqan::Tag<seqan::SmithWaterman_>]' alignment_local.cpp:65: instantiated from here /home/yyang/bin/seqan/seqan/score/score_base.h:206: error: no match for 'operator[]' in 'seq1[pos1]' /home/yyang/bin/seqan/seqan/score/score_base.h:206: error: no match for 'operator[]' in 'seq2[pos2]'