Hi Seqan Devs, I am currently working a project that used Seqan 1.4 and now I am updating to Seqan 2.1. I think that maybe I have found a bug in Seqan. The problem is that LoadReads from FragmentStore is not returning the same as in the previous version for the same call, I did a little research comparing both versions. The code of loadReads have change from one version to the other, in both versions it's defined in seqan/store/store_io.h at line 1159 for old version and at line 1273 for the new version but the behave is different and I think here is the problem. in old version there is this part: MultiSeqFile multiSeqFileL, multiSeqFileR; if (!open(multiSeqFileL.concat, toCString(fileNameL), OPEN_RDONLY)) return false; if (!open(multiSeqFileR.concat, toCString(fileNameR), OPEN_RDONLY)) return false; The flow is working correctly and the ifs don't reach the return false but in the new version the code is like this SeqFileIn seqFileL, seqFileR; if (!open(seqFileL, toCString(fileNameL)) || !open(seqFileR, toCString(fileNameR))) return false; here the execution always always return false. I inspected and its because a different open function its called and here is where I think is the bug. In seqan 1.4 the open function called is from string_map defined in /include/seqan/file/string_mmap.h however in 2.x the open function that is being called is from /include/seqan/stream/formatted_file.h. The open from string_mmap.h exists in 2.x and I think is the function that wanted to be called. LoadReads calls open with SeqFileIn in the new version while in previous with MultiSeqFile but MultiSeqFile is not available in new version Can you please check if that is a bug Thanks in Advance, Luis Enrique